An Interactive Annotated World Bibliography of Printed and Digital Works in the History of Medicine and the Life Sciences from Circa 2000 BCE to 2024 by Fielding H. Garrison (1870-1935), Leslie T. Morton (1907-2004), and Jeremy M. Norman (1945- ) Traditionally Known as “Garrison-Morton”

16061 entries, 14144 authors and 1947 subjects. Updated: December 10, 2024

Permanent Link for Entry #13159

  • 10963

Genome construction between bacterial species in vitro: Replication and expression of staphylococcus plasmid genes in Escherichia coli.

Proc. Nat. Acad. Sci. USA, 171, 1030-1034, 1974.

Confirmation of the success of methods outlined in No. 257.5.
Abstract:
"Genes carried by EcoRI endonuclease-generated fragments of Staphylococcus plasmid DNA have been covalently joined to the E. coli antibiotic-resistance plasmid pSC101, and the resulting hybrid molecules have been introduced into E. coli by transformation. The newly constructed plasmids replicate as biologically functional units in E. coli, and express genetic information carried by both of the parent DNA molecules. In addition, electron microscope heteroduplex analysis of the recombinant plasmids indicate that they contain DNA sequences derived from E. coli and Staphylococcus aureus. Recombinant molecules can transform other E. coli cells for penicillin-resistance markers originally carried by the staphylococcal plasmid, and can be transferred among E. coli strains by conjugally proficient transfer plasmids."  Digital facsimile from PubMedCentral at this link.

 



Subjects: BIOLOGY › MOLECULAR BIOLOGY › Recombinant DNA, Biotechnology, WOMEN, Publications by › Years 1900 - 1999
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