B.C.G. (Bacille Calmette–Guérin) vaccine was first produced in 1906 and subcultured for 13 years. It was first used as a prophylactic against tuberculosis in children in 1921. It remains in use. See also No. 2346.
GMN 2343 · https://historyofmedicine.com/id/3094
1927CALMETTE, Léon Charles Albert (1863 – 1933); GUÉRIN, Camille (1872 – 1961); BOQUET, Alfred (1879 – 1947); NÈGRE, Léopold (1879 – 1961)
A 250-page monograph, with bibliographical references, on the development of the BCG vaccine from M. bovis, from 1909 to 1927 by the scientists involved. (Thanks to Ron Cox for this reference.)